Present investigation was underwent on the 30 male subjects, in order to scrutinize the effect of Slow Stretch & Hold, Ballistic, and Proprioceptive Neuromuscular Facilitation Method on Hamstring Flexibility. The subjects were selected from the Department of Physical Education, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab. Only a single variable named as Hamstring flexibility was selected for the purpose of the study. The treatment i.e. Slow Stretch & Hold, Ballistic, and Proprioceptive Neuromuscular Facilitation Method was randomly assigned to all the groups (Group A, Group B, Group C). To scrutinize the effect of treatment Slow Stretch & Hold, Ballistic, and Proprioceptive Neuromuscular Facilitation Method on Hamstring flexibility “Sit and Reach Test” (AAHPERD, 1984) was induced to the subjects. Before the final commencement of the protocol on each alternate day, the subjects were asked to do proper warm up for 10 -15 minutes, followed by the active warm-up exercise from top to bottom. The subjects underwent the experiment protocols with three different Flexibility programmes i.e. Slow Stretch & Hold, Ballistic, and Proprioceptiveneuromuscular facilitation method, dispersed by a gap of alternate days in a week in the evening session for six weeks. The subjects were allowed to go through the same daily routine classes as per their curriculum on alternate day.The exercises included in Flexibility programmes were controlled in the terms of their intensity by fixing their number of repetition or duration of exercise. The obtained data was analyzed by applying Analysis of Covariance (ANCOVA), the technique was applied just to neutralize the effect of covariate (Pre-test, the results indicates that, a significant difference was found among the Slow Stretch & Hold, Ballistic and PNF Method), further to find out the critical difference s between the groups Post Hoc LSD test was employed.